Dojindo/Cell Counting Kit-8 + 96-Well Silicone Orienter/3000/CSP04,Dojindo,,Dojindo,同仁,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Dojindo/Cell Counting Kit-8 + 96-Well Silicone Orienter/3000/CSP04

产品编号：CSP04

市场价：¥8960.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$448.00

品牌： Dojindo

公司：Dojindo Molecular Technologies, Inc.

公司分类：

商品介绍

DescriptionReferencesDataQ & AManualS.D.S

Product DescriptionCell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Cell viability detection mechanism with CCK-8

ProcedureCell Counting Kit-8 requires 3 simple steps.

The handling time of Cell Counting Kit-8 is the shortest among it’s competitors. Only 15 minutes of handling time for Cell Counting Kit-8 where as longer handling time is required for both MTS and MTT assay.

StabilityCell Counting Kit-8 is a ready-to-use solution. It is also stabe at 4ºC for 1 year. Your assay can be done anytime without thaw and freeze.

SensitivityCCK-8 (WST-8) is the highest sensitive dye for the cell based assay.

Comparison of the sensitivity using tetrazolium dye

Cytotoxicity of ReagentsOnly in CCK-8, continious culture is possible without killing cells.

Observation of Cytotoxicity after incubation with each reagent.HeLa cells were incubated with WST-8 (CCK-8), WST-1, or MTS at 24 hours from addition of those reagents.

Example Data using Cell Counting Kit-8

Data for Cell Proliferation and Cytotoxicity Assay

Absorption property of WST-8The microplate reader with a 450-490 nm filter is applicable for the measurement.

Absorption spectrum of WST-8 formazan dye

How many cells should there be in a well?

For adhesive cells, at least 1,000 cells are necessary per well (100 μl medium) when using a standard 96-well plate. For leukocytes, at least 2,500 cells are necessary per well (100 μl medium) because of low sensitivity. The recommended maximum number of cells per well for the 96-well plate is 25,000. If 24-well or 6-well plates are used for this assay, please calculate the number of cells per well accordingly, and adjust the volume of the CCK-8 solution in each well to 10% of the total volume.

Can this kit be used with a 384-well plate?

Yes, you can use this kit for a 384-well plate. Add CCK-8 solution with 1/10 volume of the culture medium in a well. If the volume of CCK-8 to be added is too small, dilute CCK-8 in half with the medium and add 1/5 of the volume of culture medium in a well.

Can this kit be used with a 24-well plate?

Yes. Add CCK-8 solution with 1/10 volume of the culture medium in a well.

Does CCK-8 stain viable cells?

No, it does not stain viable cells because a water-soluble tetrazolium salt (WST-8) is used for the CCK-8 solution. The electron mediator, 1-Methoxy PMS, receives electrons from viable cells and transfers the electrons to WST-8 in the culture medium. Since its formazan dye is also highly water soluble, CCK-8 cannot be utilized for cell staining.

Does phenol red affect the assay?

No. The absorption value of phenol red in culture medium can be removed by subtracting the absorption value of a blank solution from the absorption value of each well. Therefore, culture medium containing phenol red can be used for the CCK-8 assay.

Is there a correlation between CCK-8 and the Thymidine incorporation assay?

Yes. However, please note that since CCK-8 uses a different assay mechanism from that of the Thymidine assay, the results may differ. Comparison data are shown in the technical manual, which is available at www.dojindo.com/tm.

Is CCK-8 toxic to cells?

CCK-8 solution itself is cytotoxic because of the high concentration of 1-Methoxy PMS. CCK-8 in the culture medium, however, is not cytotoxic because of the ten times dilution with culture medium. Therefore, long incubation, such as overnight and several days, is possible. The same cell culture can be used for other cell proliferation assays, such as the crystal violet assay, neutral red assay, or DNA fluorometric assays after the CCK-8 assay is completed. Since each cell has a different tolerance to CCK-8, incubate cells with CCK-8 solution and check the cell viability if a longer incubation time is necessary.

How stable is CCK-8?

CCK-8 is stable over 6 months at room temperature and one year at 0-5 oC with protection from light. For longer storage, we recommend storing the solution at -20 oC ; CCK-8 is stable over 2 years at -20^oC.

I do not have a 450 nm filter. What other filters can I use?

You can use filters with an absorbance between 450 nm and 490 nm.

Related Categories

Kits for Life Science Research

品牌介绍

Dojindo Molecular Technologies，Inc.是美国领先的生命科学研究专用试剂盒和化学品分销商。我们通过提供优质的支持和创新产品，不断扩大客户的满意度，以扩大生命科学研究和开发领域。

我们的公司文化反映出“ Jin”（仁）字符背后的含义，即尊重和关爱他人。当我们公司执行上述任务时，“ Jin”精神始终处于我们思想的最前沿。

我们如何开始

1996年

作为日本Dojindo实验室的子公司，Dojindo Molecular Technologies，Inc.在马里兰州盖瑟斯堡开业，这是新产品开发的主要设施。开发了诸如DNA损伤定量试剂盒，SOD检测试剂盒WST，标记试剂盒系列之类的产品，为全球客户提供现成的检测试剂盒。

2000

Dojindo Molecular Technologies，Inc.成为了另一个销售地点，将Dojindo产品带到了北美地区的研究专业人员手中。

2008年

我们搬迁至马里兰州罗克维尔，仅专注于北美的销售和营销活动。我们目前的位置非常接近美国国立卫生研究院（NIH），美国国家癌症研究所（NCI），FDA，约翰·霍普金斯大学的研究人员。

我们不仅向周边地区，而且向我们在美国，加拿大和南美的客户提供日本优质的客户支持和创新产品。

关于同人堂实验室

Dojindo Laboratories由螯合化学领域的著名研究人员上野敬平教授创立。同人堂实验室是1951年第一家将EDTA（乙二胺四乙酸）商业化的日本公司。自那以后，同人堂实验室一直在生产用于支持科学研究进展的试剂。Dojindo实验室的任务是开发用于生命科学研究的创新工具。Dojindo实验室致力于通过科学发现和开发新药物为改善生活质量做出贡献。