Product DescriptionHetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent
Hetero-bifunctional Reagents
| Product Name | Code | Length (Å) |
| Sulfo-EMCS | S024 | 9.4 |
| Sulfo-GMBS | S025 | 6.9 |
| Sulfo-HMCS | S026 | 13.0 |
| Sulfo-KMUS | S250 | 16.7 |
| Sulfo-SMCC | S330 | 8.0 |
