Dojindo/Lactate Assay Kit-WST/200/L256,Dojindo,,Dojindo,同仁,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Dojindo/Lactate Assay Kit-WST/200/L256

产品编号：L256

市场价：¥13940.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$697.00

品牌： Dojindo

公司：Dojindo Molecular Technologies, Inc.

公司分类：

商品介绍

DescriptionQ & AManualS.D.S

Lactate Assay Kit-WST can quantify lactate (the lower limit: 0.02 mmol/l), one of the glycolysis metabolites. Because the Lactate Assay Kit-WST corresponds to 96 well micro-plates, it allows for multiple samples to be measured at once. Lactate quantification has been widely used as a marker for monitoring changes in intracellular metabolic pathway in many different areas of cancer research. In recent years, there have been an increasing number of reports on using lactate as an indicator of stem cell differentiation or as an indicator in diabetic research.

PrincipleThe kit is used to detect lactate in cell culture medium by measuring the absorbance of WST formazan produced according to quantities of lactate. The kit includes a “Lactate Standard” which can be used to quantify the concentration of lactate found within samples by creating standard curve.

Simple Assay Procedure

Stability

Dojindo’s stock solution is stable for 4 months under refrigerator. Your assay can be done anytime without the need freeze the product and wait for defrosting.

Standard curveStandard curve can be prepared by using Lactate Standard included in the kit. The concentration of lactate can be measured. The concentration of lactate can be evaluated by diluting the samples if the concentration is over 1 mmol/l.

Can I quantify D-Lactate levels?

No, the kit is used for the quantification of L-Lactate levels.

How stable is the Working solution?

You can’t store the Working solution. Please prepare the Working solution prior to use. Please protect from light because the Working solution is light-sensitive. The Working solution is stable for 4 hours at room temperature with protection from light.Once the Working solution is exposed to light, the color of the solution becomes from red to orange. It caused to increase background.

Can I measure samples that contain a reducing agent?

You can’t measure an accurate Lactate level because WST dye interferes with the reducing agent. If you add test material which possesses reducing properties, please prepare a few wells that only contain the medium without cells and only test material for background control. Please subtract the background from the absorbance of standard curve or samples

Are there protocols for measuring intracellular lactate level?

(1) Collect cells (1 × 10⁵ cells * 1) in a microtube.(2) Centrifuge at 300 × g for 2 minutes and remove supernatant.(3) Add 300 μl of cold PBS and suspend by pipetting. Centrifuge at 300 × g for 2 minutes and remove supernatant.(4) Add 300 μl of 0.1% Triton-X * 2 and vortex for 1 minutes to prepare for cell lysate.(5) Centrifuge at 8000 × g for 5 minutes and collect supernatant.(6) Deproteinize the solution prepared at step (5) with an ultrafiltration membrane filter (fraction molecular weight: 10 K) * 3 to obtain a measurement sample.

* 1 For HeLa cells, more than 1 × 10⁵ cells are necessary to detect lactate level over 0.02 mmol / l.* 2 When SDS is included in the cell Lysate, color development can be inhibited. Please avoid using buffer containing SDS.* 3 Since endogenous Lactate dehydrogenase (LDH) causes background please remove LDH by deproteinization treatment.

How many samples can I measure?

* The number of samples that can be recorded when the standard curve and sample is measured in triplicates.

*A plate layout for Lactate standard solution and sample

I do not have a 450 nm filter. What other filters can I use?

You can use filters with an absorbance between 450 nm and 490 nm. However, the absorbance value can become lower if the sample is not measured at 450 nm.

Our samples did not change in color, are there any reasons for this?

Lactate level contained in the sample may be lower than the detection limit (0.02mmol/l) that can be measured using this kit. If the lactate level is below 0.02mmol/l, please consider other measurement method such as LC-MS.If you dilute the sample, lactate level contained in the diluted sample may be lower than 0.02mmol/l.Please lower the dilution ratio and adjust the concentration of the sample over the detection limit that was measured using this kit.

Related Categories Cell Proliferation / Cytotoxicity Intracellular Fluorescent Probes Cellular Metabolism

品牌介绍

Dojindo Molecular Technologies，Inc.是美国领先的生命科学研究专用试剂盒和化学品分销商。我们通过提供优质的支持和创新产品，不断扩大客户的满意度，以扩大生命科学研究和开发领域。

我们的公司文化反映出“ Jin”（仁）字符背后的含义，即尊重和关爱他人。当我们公司执行上述任务时，“ Jin”精神始终处于我们思想的最前沿。

我们如何开始

1996年

作为日本Dojindo实验室的子公司，Dojindo Molecular Technologies，Inc.在马里兰州盖瑟斯堡开业，这是新产品开发的主要设施。开发了诸如DNA损伤定量试剂盒，SOD检测试剂盒WST，标记试剂盒系列之类的产品，为全球客户提供现成的检测试剂盒。

2000

Dojindo Molecular Technologies，Inc.成为了另一个销售地点，将Dojindo产品带到了北美地区的研究专业人员手中。

2008年

我们搬迁至马里兰州罗克维尔，仅专注于北美的销售和营销活动。我们目前的位置非常接近美国国立卫生研究院（NIH），美国国家癌症研究所（NCI），FDA，约翰·霍普金斯大学的研究人员。

我们不仅向周边地区，而且向我们在美国，加拿大和南美的客户提供日本优质的客户支持和创新产品。

关于同人堂实验室

Dojindo Laboratories由螯合化学领域的著名研究人员上野敬平教授创立。同人堂实验室是1951年第一家将EDTA（乙二胺四乙酸）商业化的日本公司。自那以后，同人堂实验室一直在生产用于支持科学研究进展的试剂。Dojindo实验室的任务是开发用于生命科学研究的创新工具。Dojindo实验室致力于通过科学发现和开发新药物为改善生活质量做出贡献。