Dojindo/DPPH Antioxidant Assay Kit/500/D678,Dojindo,,Dojindo,同仁,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Dojindo/DPPH Antioxidant Assay Kit/500/D678

产品编号：D678

市场价：¥3800.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$190.00

品牌： Dojindo

公司：Dojindo Molecular Technologies, Inc.

公司分类：

商品介绍

DescriptionQ & AManualS.D.S

Product DescriptionChanges in antioxidant capacity of the body are involved in the development of various diseases and health problems. As a result, this increases expectations for foods with antioxidant activity (antioxidant foods). Kochi University’s Shimamura et al. have developed a method using DPPH (2,2-Diphenyl-1-picrylhydrazyl) for evaluating antioxidant activity with little difference between measurement facilities1). This product uses a method including a microplate based on the report of Shimamura et al. By manualizing the DPPH measurement method and producing a kit of stable and constant quality, we were able to reduce data variability and complexity of reagent preparation, which has experienced issues until now.1） T. Shimamura et al., Anal. Sci., 2014, 30, 717 – 721

Simple ProcedureDPPH and Trolox are unstable in solution. Please prepare immediately before using.DPPH content needs to be checked by measuring absorbance.Reagents necessary for measurement are inlcuded and subdivided. Begin experiments immediately with simple preparation before measurement.(Please use sonication to dissolve the DPPH Reagent.)

Measuring methodAfter preparing the reagent, add the reagent and sample to the 96-well microplate and react for 30 minutes.

Comparison with a conventional methodWhen measuring antioxidant activity with DPPH, the pH and solvent concentration in the solution affect the measurement. This product uses protocols and analysis methods to minimize these effects.

Effect of pHThe assay buffer provided with the product allows measurement at a certain pH.

Effect of sample solventThe sample volume is set to 1/10 (20 μL) of the total reaction solution. The kit is optimized so that there is no difference in the measured value even if the sample is dissolved in water or ethanol.

IC50 Value VariationIf the antioxidant capacity of a sample is analyzed using only IC50 values, the data will vary slightly in the different measurement conditions. By measuring the sample and standard substance (Trolox) simultaneously and calculating the antioxidant capacity as the Trolox equivalent activity value (TEAC), highly reproducible measurement values can be obtained.TEAC (μg TE/μg) = Trolox IC50 (μg/mL)/ Sample IC50 (μg/mL)”

Measurement ExampleConfirming differences between facilitiesAt three facilities, antioxidants were measured by the DPPH method.Gallic acid, catechin, and morin (known antioxidants) were measured with a spectrophotometer using a cuvette and calculated as a Trolox equivalent activity value (TEAC).There was nearly no difference in measured values between three facilities.Reference: T. Shimamura et al., NipponShokuhin Kagaku Kogaku Kaishi, 2007, 54, 482 – 487.

Comparison between microplate and cuvetteSimilar to the above experiment, three antioxidants were measured using a microplate and calculated as Trolox equivalent activity values. As a result, almost the same measurement results were obtained. This kit uses a microplate for measurement.

IC50 values vary, are there any precautions?

(1) Please make sure that all of the DPPH is dissolved.Please use sonication to dissolve the DPPH Reagent because it is difficult to dissolve.Transfer ethanol to the tube several times as described in the instruction manual. Confirm that the ethanol is no longer colored before use.(2) IC50 values may fluctuate greatly when the sample dilution interval is wide or when measured at the point where the inhibition curve is saturated (see below).Therefore, it is recommended that the optimal concentration range be confirmed in a preliminary experiment.

Can I dissolve DPPH by vortexing or pipetting?

Since DPPH is difficult to dissolve, it cannot be dissolved using vortex and pipetting.Be sure to use an ultrasonic cleaner because undissolved parts may cause variations in IC50.”

How many samples can I measure?

This depends on the kit size:

100 tests	500 tests
This kit contains enough reagents to measure one 96-well microplate (1 set of DPPH)Analyzing samples and standards in triplicate is recommended for accuracy.Therefore, the number of measurable samples is calculated as follows, assuming that measurement was performed at n: 3.DPPH Reagent per 1 kit is sufficient for 100 tests.Use for 1 sampleOne sample can be measured with the 100 test size kit. Number of wells used during preliminary experiment: Confirmation of optimum concentration range Sample: 8 (dilution series of 8 points) X 3 (n: 3) = 24 wellBlank: 1 (Blank1) X 3 (n: 3) = 3 well Number of wells used when calculating the IC50 value Sample: 8 (dilution series of 8 points) X 3 (n: 3) = 24 well Trolox: 4 (dilution series of 4 points) X 3 (n: 3) = 12 well Blank: 1 (Blank1) X 3 (n: 3)= 3 well*If there is no require preliminary experiments, the additional sample measurement is possible.	This kit contains enough reagents to measure 5 96-well microplates (5 sets of DPPH)Choose the quantity used based on your experiment (ex. 500 test x1 or 100 test x5)Up to 8 samples8 samples can be measured with the 500 test size kit.Number of wells during the preliminary experiment: Confirmation of optimum concentration rangeSample: 8 (dilution series of 8 points) X 3 (n: 3) X 8 (8 samples) = 192 wellBlank: 1 (Blank1) X 3 (n: 3) X 3 (3 plates) = 9 well Number of wells when calculating IC50 value Sample: 8 (dilution series of 8 points) X 3 (n: 3) X 8 (8 samples) = 192 welll Trolox: 4 (dilution series of 4 points) X 3 (n: 3) X 5 (5 plates) = 60 well Blank: 1 (Blank1) X 3 (n: 3) X 3 (3 plates) = 9 well

100 tests

500 tests

This kit contains enough reagents to measure one 96-well microplate (1 set of DPPH)Analyzing samples and standards in triplicate is recommended for accuracy.Therefore, the number of measurable samples is calculated as follows, assuming that measurement was performed at n: 3.DPPH Reagent per 1 kit is sufficient for 100 tests.Use for 1 sampleOne sample can be measured with the 100 test size kit.

Number of wells used during preliminary experiment: Confirmation of optimum concentration range

Sample: 8 (dilution series of 8 points) X 3 (n: 3) = 24 wellBlank: 1 (Blank1) X 3 (n: 3) = 3 well

Number of wells used when calculating the IC50 value

Sample: 8 (dilution series of 8 points) X 3 (n: 3) = 24 well

Trolox: 4 (dilution series of 4 points) X 3 (n: 3) = 12 well

Blank: 1 (Blank1) X 3 (n: 3)= 3 well*If there is no require preliminary experiments, the additional sample measurement is possible.

This kit contains enough reagents to measure 5 96-well microplates (5 sets of DPPH)Choose the quantity used based on your experiment (ex. 500 test x1 or 100 test x5)Up to 8 samples8 samples can be measured with the 500 test size kit.Number of wells during the preliminary experiment: Confirmation of optimum concentration rangeSample: 8 (dilution series of 8 points) X 3 (n: 3) X 8 (8 samples) = 192 wellBlank: 1 (Blank1) X 3 (n: 3) X 3 (3 plates) = 9 well

Number of wells when calculating IC50 value

Sample: 8 (dilution series of 8 points) X 3 (n: 3) X 8 (8 samples) = 192 welll

Trolox: 4 (dilution series of 4 points) X 3 (n: 3) X 5 (5 plates) = 60 well

Blank: 1 (Blank1) X 3 (n: 3) X 3 (3 plates) = 9 well

IC50 value is known: 3 samples・If the IC50 value has already been measured with our kit, 3 samples can be measured with one kit.・If the IC50 value is not measured with our kit, it is recommended to perform a preliminary experiment as an unknown sample.

Number of wells when calculating the IC50 valueSample: 8（dilution series of 8 points) X 3 (n: 3) X 3 (Sample number) = 72 wellTrolox: 4 (dilution series of 4 points) X 3 (n: 3) = 12 wellBlank: 1 (Blank1) X 3 (n: 3) = 3 well

If the reaction time until measurement is increase, will the measured value be affected?

Longer reaction times can change the readings.In order to get the good results with reproducibility, measure immediately after incubation (25 ° C, 30 minutes, dark place) as described in the manual.When measuring multiple plates, please unify the measurement time for each plate.

What should I do if my sample is opaque?

If the sample is clear after the pretreatment described in the manual, measurement is possible.

Related Categories Oxidative Stress Assay ACE Inhibition Assay SOD Activity, Glutathione, DNA Damage

品牌介绍

Dojindo Molecular Technologies，Inc.是美国领先的生命科学研究专用试剂盒和化学品分销商。我们通过提供优质的支持和创新产品，不断扩大客户的满意度，以扩大生命科学研究和开发领域。

我们的公司文化反映出“ Jin”（仁）字符背后的含义，即尊重和关爱他人。当我们公司执行上述任务时，“ Jin”精神始终处于我们思想的最前沿。

我们如何开始

1996年

作为日本Dojindo实验室的子公司，Dojindo Molecular Technologies，Inc.在马里兰州盖瑟斯堡开业，这是新产品开发的主要设施。开发了诸如DNA损伤定量试剂盒，SOD检测试剂盒WST，标记试剂盒系列之类的产品，为全球客户提供现成的检测试剂盒。

2000

Dojindo Molecular Technologies，Inc.成为了另一个销售地点，将Dojindo产品带到了北美地区的研究专业人员手中。

2008年

我们搬迁至马里兰州罗克维尔，仅专注于北美的销售和营销活动。我们目前的位置非常接近美国国立卫生研究院（NIH），美国国家癌症研究所（NCI），FDA，约翰·霍普金斯大学的研究人员。

我们不仅向周边地区，而且向我们在美国，加拿大和南美的客户提供日本优质的客户支持和创新产品。

关于同人堂实验室

Dojindo Laboratories由螯合化学领域的著名研究人员上野敬平教授创立。同人堂实验室是1951年第一家将EDTA（乙二胺四乙酸）商业化的日本公司。自那以后，同人堂实验室一直在生产用于支持科学研究进展的试剂。Dojindo实验室的任务是开发用于生命科学研究的创新工具。Dojindo实验室致力于通过科学发现和开发新药物为改善生活质量做出贡献。