Dojindo/ICG Labeling Kit – NH2/3/LK31,Dojindo,,Dojindo,同仁,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Dojindo/ICG Labeling Kit – NH2/3/LK31

产品编号：LK31

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Dojindo

公司：Dojindo Molecular Technologies, Inc.

公司分类：

商品介绍

DescriptionReferencesDataQ & AManualS.D.S

Product DescriptionICG Labeling Kit – NH₂ is used primarily for the preparation of ICG (Iindocyanin green)-labeled antibody for near-infrared fluorescence imaging. ICG offers two remarkable properties:

1) ICG has a strong near-infrared fluorescence even after a few days under physiological conditions. The excitation and emission wavelength of the ICG-labeled proteins are 774 nm and 805 nm, respectively.2) ICG has been used in clinical fields such as a hepatic deficiency testing. Therefore, ICG and ICG conjugates are materials suitable for in vivo imaging.

This kit contains all required compornents required for labeling, including storage buffer for conjugates. The labeling process is simple:. Add NH₂-reactive ICG to protein solution on a filter membrane, and incubate at 37ºC, for 10 minutes. A filtration tube can remove excess ICG molecules.

Fig. 1 Fluorescein Labeling Process to IgG

Precaution♦ If the IgG solution contains other proteins with molecular weight greater than 10,000, such as serum albumin or gelatin, purify the IgG solution before labeling fluorescein with this kit. Commercially available antibody may contain BSA or gelatin as a stabilizer. Dojindo offers IgG Purification Kit-A (AP01-10) and IgG Purification Kit-G (AP02-10) for the purification of the IgG solution

1. M. Ogawa, C. A. S. Regino, J. Seidel, M. V. Green, W. Xi, M. Williams, N. Kosaka, P. L. Choyke and H. Kobayashi, “Dual-Modality Molecular Imaging Using Antibodies Labeled with Activatable Fluorescence and a Radionuclide for Specific and Quantitative Targeted Cancer Detection”, Bioconjugate Chem., 2009, 20(11), 2177.2. M. Ogawa, N. Kosaka, P. L. Choyke and H. Kobayashi, “In vivo Molecular Imaging of Cancer with a Quenching Near-Infrared Fluorescent Probe Using Conjugates of Monoclonal Antibodies and Indocyanine Green”, Cancer Res., 2009, 69(4), 1268.3. N. Kosaka, M. Ogawa, P. L. Choyke and H. Kobayashi, “Clinical implications of near-infrared fluorescence imaging in cancer”, Future Oncology, 2009, 5(9), 1501.4. S. Ito, N.Muguruma, S. Hayashi, S. Taoka, T. Bando, K. Inayama, M. Sogabe, T. Okahisa, S. Okamura, H. Shibata, T. Irimura, K. Takesako and S. Shibamura, “Development of Agents for Reinforcement of Fluorescence on Near-infrared Ray Excitation for Immunohistological Staining”, Bioorg. Med. Chem., 1998, 6, 613.5. S. Ito, N. Muguruma, Y. Kakehashi, S. Hayashi, S. Okamura, H. Shibata, T. Okahisa, M. Kanamori, S. Shibamura, K. Takesako, M. Nozawa, K. Ishida and M. Shiga, “Development of Fluorescence-Emitting Antibody Labeling Substance by Near-Infrared Ray Excitation”, Bioorg. Med. Chem. Lett., 1995, 5, 2689.6. K. Inayama, S. Ito, N. Muguruma, Y. Kusaka, T. Bando, Y. Tadatsu, M. Tadatsu, K. Ii, S. Shibamura and K. Takesako, “Basic Study of an Agent for Reinforcement of Near-infrared Fluorescence on Tumor Tissue”, Digestive and Liver Disease, 2003, 35, 88.7. S. Ito, N. Muguruma, S. Hayashi, S. Taoka, T. Bando, Y. Kusaka, M. Yano, S. Ichikawa, A. Hiasa, T. Omoya, H. Honda, I. Shimizu, K. Ii, K. Nakamura, K. Takesako, Y. Goto and S. Shibamura, “Visualization of Human Gastric Cancer with a Novel Infrared Fluorescent Labeling Marker of Anti-carcinoembryonic Antigen Antibody in vitro”, Dig. Endosc., 2000, 12, 33.8. S. Taoka, S. Ito, N. Muguruma, S. Hayashi, Y. Kusaka, K. Ii, K. Nakamura, K. Imaizumi, K. Takesako and S. Shibamura, “Reflected Illumination-type Imaging System for the Development of Infrared Fluorescence Endoscopy”, Dig. Endosc.,1999, 11(4), 321.9. S. Ito, N. Muguruma, S. Hayashi, S. Taoka, A. Tsutsui, T. Fukuda, T. Okahisa, Y. Ohkita, H. Matsunaga, I. Shimizu, K. Nakamura, K. Imaizumi, K. Takesako and S. Shibamura, “Development of an Imaging System Using Fluorescent Labeling Substances Excited by Infrared Rays”, Dig. Endosc., 1997, 9, 278.10. S. Ito, N. Muguruma, Y. Kusaka, M. Tadatsu, K. Inayama, Y. Musashi, M. Yano, T. Bando, H. Honda, I. Shimizu, K. Ii, K. Takesako, H. Takeuchi and S. Shibamura, “Detection of human gastric cancer in resected specimens using a novel infrared fluorescent anti-human carcinoembryonic antigen antibody with an infrared fluorescence endoscope in vitro”, Endoscopy, 2001, 33(10), 849.11. N. Muguruma, S. Ito, T. Bando, S. Taoka, Y. Kusaka, S. Hayashi, S. Ichikawa, Y. Matsunaga, Y. Tada, S. Okamura, K. Ii, K. Imaizumi, K. Nakamura, K. Takesako and S. Shibamura, “Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays: a Novel Diagnostic Method for Micro Cancers in the Digestive Tract”, Internal Medicine, 1999, 38(7), 537.12. T. Bando, N. Muguruma, S. Ito, Y. Musashi, K. Inayama, Y. Kusaka, M. Tadatsu, K. Ii, T. Irimura, S. Shibamura and K. Takesako, “Basic Study on a Labeled anti-mucin Antibody Detectable by Infrared-fluorescence Endoscopy”, J. Gastroenterol., 2002, 37, 260.13. N. Muguruma, S. Ito, S. Hayashi, S. Taoka, H. Kakehashi, K. Ii, S. Shibamura and K. Takesako, “Antibodies Labeled with Fluorescence-agent Excitable by Infrared Rays”, J. Gastroenterol., 1998, 33, 467.14. W. Aung, A. Tsuji, H. Sudo, A. Sugyo, T. Furukawa, Y. Ukai, Y. Kurosawa and T. Saga, “Immunotargeting of Integrin α6β4 for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model”, Molecular Imaging, 2016, 15, 1.

Fig. 3 Fluorescent Property of ICG Dye

Can I use this kit for F(ab")₂?

Yes, please follow the labeling protocol for IgG. The recovery of the conjugate should be over 80%.

Can I use this kit for other proteins or peptides?

Yes, if the molecular weight of the reduced form is greater than 50,000 or less than 5,000, and it has a reactive SH group, or a disulfide group that can be reduced without losing activity. If the molecular weight is greater than 50,000, follow the labeling protocol for IgG, and use 0.5-1 nmol of sample protein for LK09-10. If the molecular weight is less than 5,000, follow the labeling protocol for small molecules. If the molecular weight is between 5,000 and 50,000, contact our customer service at info@dojindo.com or 1-877-987-2667 for more information.

Can I use this kit to label oligopeptides or oligonucleotides?

Yes, if the molecular weights of the oligonucleotide or the oligopeptide are less than 5,000 and they have at least one SH group. Follow the labeling protocol for small molecule.

What is the minimum amount of IgG that can be labeled with LK13-10?

The minimum amount is 50 μg. There is no significant difference in sensitivity and background between 50 μg and 200 μg of IgG. However, even 10 μg IgG can be labeled using 1/5 volume of SH-reactive peroxidase solution at Step 8.

How many alkaline phosphatase molecules per reduced IgG are introduced?

The average number of alkaline phosphatase molecule per reduced IgG is 1 to 2.

Do I have to use a Filtration tube prior to labeling the protein?

If the protein solution does not contain small molecules with reactive SH groups and the concentration of the protein is 10 mg per ml, or about 70 μM, there is no need to use the Filtration tube. Just mix 10 μl of the sample solution with Solution B and add the mixture to a vial of the SH-reactive peroxidase.

Do I have to use Storage buffer included with the kit?

No, you don’t have to use Storage buffer from the kit. You can choose any kind of buffer appropriate for your experiment.

My sample contains small insoluble material. What should I do?

Spin the sample and use the supernatant for labeling.

Does unconjugated SH-reactive ALP still have a reactive maleimide after the labeling reaction to IgG?

No. Nearly 100% of SH-reactive ALP is used for the IgGlabeling or the small molecule labeling.

Does Storage buffer contain animal products or polymers?

No, Storage buffer does not contain any animal products , polymers, or heavy metal ions.

Related Categories Labeling Chemistry

品牌介绍

Dojindo Molecular Technologies，Inc.是美国领先的生命科学研究专用试剂盒和化学品分销商。我们通过提供优质的支持和创新产品，不断扩大客户的满意度，以扩大生命科学研究和开发领域。

我们的公司文化反映出“ Jin”（仁）字符背后的含义，即尊重和关爱他人。当我们公司执行上述任务时，“ Jin”精神始终处于我们思想的最前沿。

我们如何开始

1996年

作为日本Dojindo实验室的子公司，Dojindo Molecular Technologies，Inc.在马里兰州盖瑟斯堡开业，这是新产品开发的主要设施。开发了诸如DNA损伤定量试剂盒，SOD检测试剂盒WST，标记试剂盒系列之类的产品，为全球客户提供现成的检测试剂盒。

2000

Dojindo Molecular Technologies，Inc.成为了另一个销售地点，将Dojindo产品带到了北美地区的研究专业人员手中。

2008年

我们搬迁至马里兰州罗克维尔，仅专注于北美的销售和营销活动。我们目前的位置非常接近美国国立卫生研究院（NIH），美国国家癌症研究所（NCI），FDA，约翰·霍普金斯大学的研究人员。

我们不仅向周边地区，而且向我们在美国，加拿大和南美的客户提供日本优质的客户支持和创新产品。

关于同人堂实验室

Dojindo Laboratories由螯合化学领域的著名研究人员上野敬平教授创立。同人堂实验室是1951年第一家将EDTA（乙二胺四乙酸）商业化的日本公司。自那以后，同人堂实验室一直在生产用于支持科学研究进展的试剂。Dojindo实验室的任务是开发用于生命科学研究的创新工具。Dojindo实验室致力于通过科学发现和开发新药物为改善生活质量做出贡献。