Dojindo/R-Phycoerythrin Labeling Kit-NH2/3/LK23,Dojindo,,Dojindo,同仁,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Dojindo/R-Phycoerythrin Labeling Kit-NH2/3/LK23

产品编号：LK23

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Dojindo

公司：Dojindo Molecular Technologies, Inc.

公司分类：

商品介绍

DescriptionReferencesQ & AManualS.D.S

Product DescriptionPhycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. R-Phycoerythrin (R-PE) is one of the phycobiliproteins and has a red fluorescence at around 578 nm, and it can be excited at 488nm (Fig. 1). Because of this high fluorescence, phycobiliprotein labeled antibodies, and other molecules can provide greater sensitivity in flow cytometry and immunostaining. R-phycoerythrin Labeling Kit-NH₂ is for simple and rapid preparation of R-PE-labeled IgG (Fig. 2). NH₂-reactive R-PE (a component of this kit) has an activated ester group and can easily make a covalent bond with an amino group of the target molecule without any activation process. The filtration tube in this kit allows a quick buffer exchange and concentration of sample IgG solution. This kit contains all of the reagents necessary for R-PE labeling, including the Storage buffer for conjugates.

Fig. 1 Fluorescence spectrum of R-PEExcitation wavelength: 566 nmEmission wavelength: 578 nm

Fig. 2 IgG labeling reaction of NH₂-reactive R-PE

Precaution♦ The molecular weight of the protein to be labeled with this kit should be greater than 50,000.♦ IgG or R-phycoerythrin-conjugated IgG is always on the membrane of the filtration tube during the labeling process.♦ If the IgG solution contains other proteins with molecular weight larger than 10,000, such as BSA or gelatin, purify the IgG solution prior to labeling R-phycoerythrin with this kit. IgG solution can be purified by IgG Purification Kits (not included in this kit).♦ If the IgG solution contains small insoluble materials, centrifuge the solution and use the supernatant for the labeling.

1. K.R. McCarthy, A. Watanabe, M. Kuraoka, K.T. Do, C.E. McGee, G.D. Sempowski, T.B. Kepler, A.G. Schmidt, G. Kelsoe and S.C. Harrison, “Memory B Cells that Cross-React with Group 1 and Group 2 Influenza A Viruses Are Abundant in Adult Human Repertoires”, Immunity., 2018, 48, (1), 174.2. K.Y Su, A. Watanabe, C.H. Yeh, G. Kelsoe, M. Kuraoka, “Efficient Culture of Human Naive and Memory B Cells for Use as APCs”, J. Immunol.., 2016, 197, (10), 4163.3. T. Tsujikawa, T. Yaguchi, G. Ohmura, S. Ohta, A. Kobayashi, N. Kawamura, T. Fujita, H. Nakano, T. Shimada, T. Takahashi, R. Nakao, A. Yanagisawa, Y. Hisa, Y. Kawakami, “Autocrine and paracrine loops between cancer cells and macrophages promote lymph node metastasis via CCR4/CCL22 in head and neck squamous cell carcinoma”, Int. J. Cancer., 2013, 132, (12), 2755.4. Y. Inaguma, Y. Akahori, Y. Murayama, K. Shiraishi, S. Tsuzuki-Iba, A. Endoh, J. Tsujikawa, A. Demachi-Okamura, K. Hiramatsu, H. Saji, Y. Yamamoto, N. Yamamoto, Y. Nishimura, T. Takahashi, K. Kuzushima, N. Emi, Y. Akatsuka, “Construction and molecular characterization of a T-cell receptor-like antibody and CAR-T cells specific for minor histocompatibility antigen HA-1H”, Gene Ther.., 2014, 21, (6), 575.

Can I use this kit for other proteins?

Yes, if the molecular weight is higher than 50,000, and it has a reactive primary or secondary amino group. Follow the protocol for IgG labeling with 0.5-1 nmol of sample protein.

How many R-PE molecules per IgG are introduced?

The average number of R-PE molecule per IgG is 1 to 2.

Does unconjugated NH₂-reactive R-PE still have an activated ester after the labeling reaction to IgG?

No. It is completely hydrolyzed during the reaction.

Does NH₂-reactive R-PE form an oligomer during the labeling reaction?

No. Since all amino groups of NH₂-reactive R-PE are blocked, no oligomerization is possible.

What is the minimum amount of IgG that can be labeled with LK23-10?

The minimum amount is 50 μg. There is no significant difference in sensitivity and background between 50 μg and 200 μg of IgG.

Do I have to use WS buffer included with the kit?

Yes. Use the WS buffer to prepare a stock solution of the conjugate. However, you can choose any kind of buffer appropriate to dilute the conjugate stock solution for your experiment.

How long is the conjugate stable?

If you store at 4ºC, it is stable for over 2 months. For longer storage, add 100% volume of glycerol, aliquot, and store at -20ºC. However, please note that the stability depends on the protenitself.

Related Categories Labeling Chemistry

品牌介绍

Dojindo Molecular Technologies，Inc.是美国领先的生命科学研究专用试剂盒和化学品分销商。我们通过提供优质的支持和创新产品，不断扩大客户的满意度，以扩大生命科学研究和开发领域。

我们的公司文化反映出“ Jin”（仁）字符背后的含义，即尊重和关爱他人。当我们公司执行上述任务时，“ Jin”精神始终处于我们思想的最前沿。

我们如何开始

1996年

作为日本Dojindo实验室的子公司，Dojindo Molecular Technologies，Inc.在马里兰州盖瑟斯堡开业，这是新产品开发的主要设施。开发了诸如DNA损伤定量试剂盒，SOD检测试剂盒WST，标记试剂盒系列之类的产品，为全球客户提供现成的检测试剂盒。

2000

Dojindo Molecular Technologies，Inc.成为了另一个销售地点，将Dojindo产品带到了北美地区的研究专业人员手中。

2008年

我们搬迁至马里兰州罗克维尔，仅专注于北美的销售和营销活动。我们目前的位置非常接近美国国立卫生研究院（NIH），美国国家癌症研究所（NCI），FDA，约翰·霍普金斯大学的研究人员。

我们不仅向周边地区，而且向我们在美国，加拿大和南美的客户提供日本优质的客户支持和创新产品。

关于同人堂实验室

Dojindo Laboratories由螯合化学领域的著名研究人员上野敬平教授创立。同人堂实验室是1951年第一家将EDTA（乙二胺四乙酸）商业化的日本公司。自那以后，同人堂实验室一直在生产用于支持科学研究进展的试剂。Dojindo实验室的任务是开发用于生命科学研究的创新工具。Dojindo实验室致力于通过科学发现和开发新药物为改善生活质量做出贡献。